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Blank control Sample Assay buffer 40ul 40ul Sample to be measured 0ul 50ul Lysate 50ul 0ul Ac-YVAD-pNA (2 mM) 10ul 10ul Total Volume 100ul 100ul
5) Read the fluorescent signal on a fluorescence microplate reader [Note 5]
thereby assembling the necrosome complex and triggering membrane rupture
Add 100μL of biotinylated detection antibody at working concentration to each well of the standard well and sample well
and HRP enzyme conjugate were sequentially added to microwells pre-coated with mouse type I collagen cross-linked carboxy terminal peptide (CTX I) antigen
Alexa Fluor® 488 Rat Anti-Mouse CD41 Antibody (S-R690) Size:100T Blank control Sample Assay bufferProduct Specification Host Rat Antigen CD41 Synonyms Integrin alpha IIb; GPalpha IIb (GPIIb); Platelet membrane glycoprotein IIb; Itga2b Location Membrane Accession Q9QUM0 Clone Number S R690 Antibody Type Rat mAb Isotype IgG1,k Application FCM Reactivity Ms Positive Sample C57BL 6 mouse platelets Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300
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