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Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis
Wash the harvested cells three times with pre-chilled PBS and resuspend in 150-200µL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS
affecting protein activity and function
Concentrated wash (25 ×)
releasing cell contents to the blood
Rat SIRT3 ELISA Kit DNA Immunoprecipitation Blockade of LOX-1 functions mayProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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