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11Add 100ul HRP enzyme labeled anti mouse & Rabbit secondary antibody polymer was incubated for 30 min Note: this liquid is sensitive to light
such as oxidized low-density lipoprotein (oxLDL)
Centrifugation at 15000×g for 20 min at 4 ° C
extend the incubation time and color development time Serial number Possible cause Solutions 1 Improper storage of kits
by SDS-PAGE under reducing conditions Endotoxin <1EU/μg Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer 20mM Tris-HCl
PD-L2/B7-DC Fc Chimera Protein, Cynomolgus Size:100μg 11Add 100ul HRP enzyme labeledProduct Specification Species Cynomolgus Synonyms PD L2, B7 DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2 Accession XP_005581839 Amino Acid Sequence Leu20 Pro219, with C terminal Human IgG1 Fc
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