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the cells were incubated overnight in a 37 ° C incubator without CO2until they stained blue
(3) according to the following table in order to wash finish adding volume of corresponding reagent EP tube
and placental trophoblast cells
it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min
repeat 3 Secondary
Human RBP4 , His tag Size:100μg the cells were incubated overnightProduct Specification Species Human Synonyms Plasma retinol binding protein (PRBP; RBP) Accession P02753 Amino Acid Sequence Protein sequence(P02753, Glu19 Leu201, with C 10*His) ERDCRVSSFRVKENFDKARFSGTWYAMAKKDPEGLFLQDNIVAEFSVDETGQMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIVDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPPEAQKIVRQRQEELCLARQYRLIVHNGYCDGRSERNLLGGGGSHHHHHHHHHH Expression System HEK293 Molecular Weight Theoretical: 22. 7kDa Actual: 24kDa